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研究業績PUBLICATION

論文

2018


Yutaka Saito, Misaki Oikawa, Hikaru Nakazawa, Teppei Niide, Tomoshi Kameda, Koji Tsuda, and Mitsuo Umetsu
Machine-Learning-Guided Mutagenesis for Directed Evolution of Fluorescent Proteins
ACS Synthetic Biology, in press. DOI: 10.1021/acssynbio.8b00155

Kyongwan Kim, Natsuhiko Yoshinaga, Sanjib Bhattacharyya, Hikaru Nakazawa, Mitsuo Umetsu, Winfried Teizer
Large-scale chirality in an active layer of microtubules and kinesin motor proteins
Soft Matter, 14(17), 3221-3231 (2018). DOI: 10.1039/C7SM02298K

Hikaru Nakazawa, Yasuko Seta, Tatsuya Hirose, Yoshitake Masuda, and Mitsuo Umetsu
Use of a Phage-Display Method to Identify Peptides That Bind to a Tin Oxide Nanosheets
Protein & Peptide Letters, 25, 68-75 (2018). DOI: 10.2174/0929866525666171206114429

Hideaki Sanada, Kazuki Kobayashi, Kenji Oyama, Takamitsu Maru, Takeshi Nakanishi, Mitsuo Umetsu, Ryutaro Asano, and Izumi Kumagai
Affinity maturation of humanized anti-epidermal growth factor receptor antibody using a modified phage-based open sandwich selection method Scientific Reports, 8, 5414 (2018). April 03, DOI: 10.1038/s41598-018-23796-3

Hiroto Fujii, Yoshikazu Tanaka, Hikaru Nakazawa, Aruto Sugiyama, Noriyoshi Manabe, Akira Shinoda, Nobutaka Shimizu, Takamitsu Hattori, Katsuhiro Hosokawa, Takuma Sujino, Tomoyuki Ito, Teppei Niide, Ryutaro Asano, Izumi Kumagai, Mitsuo Umetsu.
Compact Seahorse‐Shaped T Cell–Activating Antibody for Cancer Therapy.
Advanced Therapeutics 1700031 (2018). DOI: 10.1002/adtp.201700031

Asano R, Nagai K, Makabe K, Takahashi K, Kumagai T, Kawaguchi H, Ogata H, Arai K, Umetsu M, Kumagai I.
Structural considerations for functional anti-EGFR × anti-CD3 bispecific diabodies in light of domain order and binding affinity.
Oncotarget 9(17): 13884-13893 (2018). DOI: 10.18632/oncotarget.2449

Zuzana Konvičková, Veronika Holišová, Marek Kolenčík, Teppei Niide, Gabriela Kratošová, Mitsuo Umetsu, Jana Seidlerová
Phytosynthesis of colloidal Ag-AgCl nanoparticles mediated by Tilia sp. leachate, evaluation of their behaviour in liquid phase and catalytic propertie
Colloid and Polymer Science, 296, 677–687 (2018). DOI: https://doi.org/10.1007/s00396-018-4290-2

Aruto Sugiyama, Mitsuo Umetsu, Hikaru Nakazawa, Teppei Niide, Ryutaro Asano, Hattori Takamitsu, Izumi Kumagai
High-throughput cytotoxicity and antigen-binding assay for screening small bispecific antibodies without purification
Journal of Bioscience and Bioengineering, 17:31237-31239 (2018). DOI: doi.org/10.1016/j.jbiosc.2018.02.007

Saito R, Saito Y, Nakazawa H, Hattori T, Kumagai I, Umetsu M, Makabe K.
Impact in stability during sequential CDR grafting to construct camelid VHH antibodies against zinc oxide and gold.
J Biochem. 164(1), 21-25 (2018). DOI: 10.1093/jb/mvy016

2017

Aruto Sugiyama, Mitsuo Umetsu, Hikaru Nakazawa, Teppei Niide, Tomoko Onodera, Katsuhiro Hosokawa, Shuhei Hattori, Ryutaro Asano, Izumi Kumagai
A semi high-throughput method for screening small bispecific antibodies with high cytotoxicity
Scientific Reports, 7:2862, 1-12 (2017) DOI:10.1038/s41598-017-03101-4


Nakazawa H, Seta Y, Hirose T, Masuda Y, Umetsu M.
Use of a Phage-Display Method to Identify Peptides That Bind to a Tin Oxide Nanosheets.
Protein Pept Lett. 25: (2017) DOI: 10.2174/0929866525666171206114429

2016

Masao Nakayama, Ryohei Sasaki, Chiaki Ogino, Tsutomu Tanaka, Kenta Morita, Mitsuo Umetsu, Satoshi Ohara, Zhenquan Tan, Yuya Nishimura, Hiroaki Akasaka, Kazuyoshi Sato, Chiya Numako, Seiichi Takami and Akihiko Kondo,
“Titanium peroxide nanoparticles enhanced cytotoxic effects of X-ray irradiation against pancreatic cancer model through reactive oxygen species generation in vitro and in vivo”,
Radiation Oncology, 11:91 (2016). DOI: 10.1186/s13014-016-0666-y


Yuki Shibuya, Natsuki Haga, Ryutaro Asano, Hikaru Nakazawa, Takamitsu Hattori, Daisuke Takeda, Aruto Sugiyama, Reiko Kurotani, Izumi Kumagai, Mitsuo Umetsu, and Koki Makabe,
“Generation of camelid VHH bispecific constructs via in-cell intein-mediated protein trans-splicing”,
Protein Engineering, Design & Selection, 30(1), 15-21 (2016). DOI: doi.org/10.1093/protein/gzw057

Yuki Shibuya, Natsuki Haga, Ryutaro Asano, Hikaru Nakazawa, Takamitsu Hattori, Daisuke Takeda, Aruto Sugiyama, Reiko Kurotani, Izumi Kumagai, Mitsuo Umetsu, and Koki Makabe
“Generation of camelid VHH bispecific constructs via in-cell intein-mediated protein trans-splicing”
Protein Engineering, Design & Selection, 30(1), 15-21 (2016). DOI: doi.org/10.1093/protein/gzw057

Kyongwan Kim, Aurelien Sicora, Hikaru Nakazawa, Mitsuo Umetsu, Wonmuk Hwang, Winfried Teizer
Isomorphic coalescence of aster cores formed in vitro from microtubules and kinesin motors
Physical Biology, 13(5), 056002 (2016). DOI: 10.1088/1478-3975/13/5/056002

Yutaro Mori, Hikaru Nakazawa, G. A. L. Gonçalves, Tsutomu. Tanaka, Mitsuo. Umetsu, and Noriho Kamiya,
“One-dimensional assembly of functional proteins: toward design of an artificial cellulosome”
Molecular Systems Design & Engineering, 1, 66-73 (2016). DOI: 10.1039/c5me00011d.

Ryutaro Asano, Noriaki Koyama, Yasuyo Hagiwara, Yosuke Masakari, Ryota Orimo, Kyoko Arai, Hitomi Ogata, Shozo Furumoto, Mitsuo Umetsu, and Izumi Kumagai
Anti-EGFR scFv tetramer (tetrabody) with a stable monodisperse structure, strong anticancer effect, and long in vivo half-life
FEBS Open Bio, 594-598.

Takahara M, Budinova GA, Nakazawa H, Mori Y, Umetsu M, Kamiya N.
Salt-Switchable Artificial Cellulase Regulated by a DNA Aptamer.
Biomacromolecules. (2016) DOI: 10; 17(10):3356-3362.


梅津 光央, “タンパク質工学の先頭走者:抗体医薬”, 48 (4), 2-3(156-157)
(2016) 月刊「細胞」, 2016年4月号 (2016.4)

志波 公平, 舩戸 美幸, 廣瀬 雅子, 梅津 光央,
“バイオ医薬品の開発に向けた多角的物性解析”, 月刊「細胞」, 48 (4),
36-39(190-193) (2016) 2016年4月号 (2016.4)

梅津 光央,
“ナノとタンパク質のいい関係:利点を相補完するハイブリッドタンパク質設計”,
化学と生物, 54, 240-242 (2016.3)


2015

Daniel M. Packwooda, Kazuto Akagi, Mitsuo Umetsu
“Identification of Peptide Adsorbates for Strong Nanoparticle-Nanoparticle Binding by Lattice Protein Simulations”
Materials Discovery, 1, 2-9


Niide T, Ozawa K, Nakazawa H, Oliveira D, Kasai H, Onodera M, Asano R, Kumagai I, Umetsu M.
"Organic crystal-binding peptides: morphology control and one-pot formation of protein-displaying organic crystals."
Nanoscale, 7, 20155–20163 (2015). DOI: 10.1039/c5nr06471f


Aurelien Sikora, Filippo Federici Canova, Kyongwan Kim, H
ikaru Nakazawa, Mitsuo Umetsu, Izumi Kumagai, Tadafumi Adschiri, Wonmuk Hwang, and Winfried Teizer
“Behavior of Kinesin Driven Quantum Dots Trapped in a Microtubule Loop”
ACS Nano, 9 (11), 11003–11013 (2015). DOI: 10.1021/acsnano.5b04348

Ryosuke Sasaki, Soichiro Kitazawa, Ryo Kitahara, Hikaru Nakazawa, Yoshikazu Tanaka, Izumi Kumagai, Mitsuo Umetsu, and Koki Makabe
“Zinc ion-binding activity of an anti-ZnO VHH antibody, 4F2”
Chemistry letters, 44(10), 1309-1311 (2015). DOI: 10.1246/cl.150537

Aurélien Sikora, Javier Ramón-Azcón, Mustafa Sen, Kyongwan Kim, Hikaru Nakazawa, Mitsuo Umetsu, Izumi Kumagai, Hitoshi Shiku, Tomokazu Matsue, and Winfried Teizer
“Microtubule guiding in a multi-walled carbon nanotube circuit”
Biomedical Microdevices, 17, 78 (1-6)

Kyongwan Kim, Aurélien Sikora, Koji S. Nakayama, Mitsuo Umetsu, Wonmuk Hwang, and Winfried Teizer
“Electric field-induced reversible trapping of microtubules along metallic glass microwire electrodes”
Journal of Applied Physics, 117, 144701 (2015) doi: 10.1063/1.4917203


2014

Kyongwan Kim, Aurélien Sikora, Koji S. Nakayama, Hikaru Nakazawa, Mitsuo Umetsu, Wonmuk Hwang, and Winfried Teizer
“Functional localization of kinesin/microtubule-based motility system along metallic glass microwires”
Applied Physics Letters, 105, 143701 (2014). doi: 10.1063/1.4896964


Kyongwan Kim, Andrew L. Liao, Aurélien Sikora, Daniel Oliveira, Hikaru Nakazawa, Mitsuo Umetsu, Izumi Kumagai, Tadafumi Adschiri, Wonmuk Hwang, and Winfried Teizer
“Microtubule shuttles on kinesin-coated glass micro-wire tracks”
Biomedical Microdevices, 16, 501-508


Aurélien Sikora, Javier Ramón-Azcón, Kyongwan Kim, Kelley Reaves, Hikaru Nakazawa, Mitsuo Umetsu, Izumi Kumagai, Tadafumi Adschiri, Hitoshi Shiku, Tomokazu Matsue, Wonmuk Hwang, and Winfried Teizer
"Molecular Motor-Powered Shuttles along Multi-walled Carbon Nanotube Tracks"
Nano Letters, 47, 876–881 (2014). DOI: 10.1021/nl4042388


2013

Zhenquan Tan, Kazuyoshi Sato, Seiichi Takami, Chiya Numako, Mitsuo Umetsu, Kohei Soga, Masao Nakayama, Ryohei Sasaki, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo, Kazuhiro Yamamoto, Takeshi Hashishin, Satoshi Ohara
“Particle size for photocatalytic activity of anatase TiO2 nanosheets with highly exposed {001} facets”
RSC Advances, 3, 19268-19271


Hikaru Nakazawa, Do-Myoung Kim, Takeshi Matsuyama, Nobuhiro Ishida, Akinori Ikeuchi, Yuri Ishigaki, Izumi Kumagai, and Mitsuo Umetsu
"Hybrid nanocellulosome design from cellulase modules on nanoparticles: Synergistic effect of catalytically divergent cellulase modules on cellulose degradation activity”
ACS Catalysis, 3, 1342-1348 (2013). DOI: 10.1021/cs400012v


Hikaru Nakazawa, Rui Todokoro, Yuri Ishigaki, Izumi Kumagai, and Mitsuo Umetsu
“In-one-pot-at-a-time ligation for high-throughput construction of a protein expression vector library”
Chemistry letters, 42, 424-426 (2013).


Hikaru Nakazawa, Akinori Ikeuchi, Do-Myoung Kim, Yuri Ishigaki, Hidetaka Asano, Katsunori Kouda, Izumi Kumagai, and Mitsuo Umetsu
“Biomass-binding peptides designed by molecular evolution for efficient degradation of cellulose in biomass by cellulase
, Green Chemistry, 15, 365-369 (2013).


Keiko Tawa, Mitsuo Umetsu, Hikaru Nakazawa, Takamitsu Hattori, and Izumi Kumagai
“Application of 300x enhanced-fluorescence on a plasmonic chip modified with bispecific antibody to a sensitive immunosensor”
ACS Applied Materials & Interfaces, 5, 8628-8632 (2013).


Keiko Tawa, Mari Satoh, Koichi Uegaki, Tomoko Hara, Masami Kojima, Hiroyuki Aota, Yoshiki Yokota, Takahiko Nakaoki, Mitsuo Umetsu, Hikaru Nakazawa, and Izumi Kumagai
“Rapid and sensitive detection of brain-derived neurotrophic factor (BDNF) with a plasmonic chip”
Japanese Journal of Applied Physics, 52, 6GK01 (2013).


Ryutaro Asano, Yasuyo Hagiwara, Noriaki Koyama, Yosuke Masakari, Ryota Orimo, Kyoko Arai, Hiromi Ogata, Shozo Furumoto, Mitsuo Umetsu, and Izumi Kumagai
“Multimerization of anti-(epidermal growth factor receptor) IgG fragments induces an antitumor effect: the case for humanized 528 scFv multimers”
FEBS Journal, 280, 4816–4826 (2013). DOI: 10.1111/febs.12451


Ryutaro Asano, Takashi Kumagai, Keisuke Nagai, Shintaro Taki, Ippei Shimomura, Kyoko Arai, Hiromi Ogata, Mai Okada, F. Hayasaka, H. Sanada, Takeshi Nakanishi, T. Karvonen, H. Hayashi, Y. Katayose, M. Unno, T. Kudo, Mitsuo Umetsu, and Izumi Kumagai, “Domain order of a bispecific diabody dramatically enhances its antitumor activity beyond structural format conversion: the case of the hEx3 diabody”, Protein Engineering Design & Selection, 26(5), 359-367 (2013).

Takeshi Nakanishi, Takamitsu Maru, Kazuhiro Tahara, Hideaki Sanada, Mitsuo Umetsu, Ryutaro Asano, and Izumi Kumagai
“Development of an affinity-matured humanized anti-epidermal growth factor receptor antibody for cancer immunotherapy”
Protein Engineering Design & Selection, 26, 113–122 (2013)
.


梅津 光央, 熊谷 泉
新規巻き戻し法による標的タンパク質の調製 ―完全変性を経由しない巻き戻し法―
BIO INDUSTRY, 30, 29-34 (2013).

青木 宣明, 冨樫 貴成, 北條 大介, 高見 誠一, 梅津 光央, 名嘉  節, 阿尻 雅文
超臨界法による有機修飾ナノ粒子合成と有機無機ハイブリッド材料創成
日本接着学会誌, 49, 191-196 (2013).

梅津 光央
ポストゲノム時代のスマートバイオデザイン: セルロース糖化酵素を例に
化学工学会東北支部NEWS LETTER, 80,10-13, (2013).


著書

梅津 光央, 嶋田 友一郎, 服部 峰充
『生物模倣材料と新材料・新製品開発への応用』 第5章 第5節 無機親和性ペプチドを利用したハイブリッドナノアセンブリ材料
技術情報協会, 352-358 (2014)

梅津 光央, 中澤 光, 服部 峰充
『進化分子工学』 第1編 第1章 構造情報と進化工学的アプローチがもたらした建設的タンパク質設計
エヌ・ティー・エス, 27-37 (2013).


総説・解説

学会誌など

青木 宣明, 冨樫 貴成, 北條 大介, 高見 誠一, 梅津 光央, 名嘉 , 阿尻 雅文
超臨界法による有機修飾ナノ粒子合成と有機無機ハイブリッド材料創成
日本接着学会誌, 49(5), 191-196 (2013)

その他

梅津 光央, 中澤 光, 二井手 哲平
”構造情報を取り入れた進化工学的アプローチによる分子認識タンパク質の設計”
MEDCHEM NEWS, 27(1), 25-29 (2017.2)

梅津光央
“タンパク質工学の先頭走者:抗体医薬”
月刊「細胞」, 2016年4月号 (2016.4)

志波 公平, 舩戸 美幸, 廣瀬 雅子, 梅津 光央
“バイオ医薬品の開発に向けた多角的物性解析”
月刊「細胞」, 2016年4月号 (2016.4)

梅津 光央
“ナノとタンパク質のいい関係:利点を相補完するハイブリッドタンパク質設計”
化学と生物, 54, 240-242 (2016.3)

梅津 光央
“抗体を取得してから標的を特定する”
トピックス紹介, 化学工学会バイオ部会ニュースレター, 41, 18 (2016.2)

梅津 光央
“セルロース分解に新たな役者が登場”
トピックス紹介, 化学工学会バイオ部会ニュースレター, 40, 18 (2015.10)

梅津 光央
“抗体は有機分子化していくか?”
トピックス紹介, 化学工学会バイオ部会ニュースレター, 39, 18 (2015.6)

梅津 光央
“酵素を固定化させる最近の魅力”
トピックス紹介, 化学工学会バイオ部会ニュースレター, 38, 19-20 (2015.2)

梅津 光央
“インタラクティブバイオ界面”
トピックス紹介, 化学工学会バイオ部会ニュースレター, 37, 14 (2014.10)

梅津 光央, 中澤 光
“ナノの発想からの新しい酵素複合体設計:ハイブリッドナノセルロソーム”
トピックス, 酵素工学ニュース, 酵素工学研究会, 72, 10-14 (2014.10)

梅津 光央
“抗体を多価・多重化する”
トピックス紹介, 化学工学会バイオ部会ニュースレター, 36 (2014)

梅津 光央, 熊谷 泉
“新規巻き戻し法による標的タンパク質の調製―完全変性を経由しない巻き戻し法―”
月刊「BIO INDUSTRY」, 30(7), 29-34 (2013)

梅津 光央, 服部 峰充, 熊谷 泉
“ナノ材を標的とする抗体によるナノ粒子接合”
トピックス, 高分子, 62(2), 83-84 (2013)

梅津 光央
“ポストゲノム時代のスマートバイオデザイン: セルロース糖化酵素を例に”
化学工学会東北支部NEWS LETTER, 80, 10-13 (2013)

受賞歴

2018

杉山 在生人 (D3)
東北大学 工学研究科長賞

2016

服部 修平 (M2)
東北大学 工学研究科長賞


杉山 在生人 (D2)
Job Fair 2016
Job Fair 2016最優秀賞

杉山 在生人 (D2)
第68回日本生物工学会大会
若手会口頭発表優秀賞

杉山 在生人 (D2)
The Tohoku University's Chemistry Summer School
Poster Award

服部 修平 (M2)
生物工学若手研究者の集い夏のセミナー2016
優秀ポスター賞


2015

服部峰充(特任助教)
第15回日本蛋白質科学会年会
若手奨励賞優秀賞

杉山在生人(D1)
2015 International Symposium on Chemical and Polyscale Technologies for Biomedical Application and Environmental
Out Standing Oral Award

杉山在生人(D1)
第15回日本蛋白質科学会年会
日本蛋白質科学会ポスター賞

中山純一(M1)
The Tohoku University's Chemistry Summer School
Poster Award


2014

藤井滉人(M1)
生体機能関連化学部会若手の会 第26回サマースクール
優秀ポスター賞


2012

外所瑠衣(M1)
The 18th Symposium of Young Asian Biochemical Engineer's Community
Poster Award



2012年以前の業績